Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection

Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection

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Background: Herpes simplex virus (HSV) is usually dormant and becomes apparent when body conditions decline.We investigated the anti-HSV activity of various natural and synthetic compounds for future clinical application.Methods: Mock- and HSV-infected Vero cells were treated for three days with various concentrations HORNY GOAT WEED of samples.For short exposure, 100-fold concentrated virus were preincubated for 3 min with samples, diluted to normal multiplicity of infection (MOI), before the addition to the cells.

Anti-HSV activity was evaluated by the chemotherapy index.Results: Alkaline extracts of the leaves of Sasa sp.(SE) and pine cone (PCE) showed higher anti-HSV activity than 20 Japanese traditional herb medicines (Kampo formulas), four popular polyphenols, and 119 chromone-related compounds.Exposure of HSV to SE or PCE for 3 min almost completely eliminated the infectivity of HSV, whereas much longer exposure time was required for Kakkonto, the most active Kampo formulae.

Anti-HSV activity of PCE and Kakkonto could be detected only when they were dissolved by alkaline solution (pH 8.0), but not by neutral buffer (pH 7.4).Anti-HSV activity of SE and povidone iodine was stable if they were diluted with neutral buffer.

Conclusions: The present study suggests the applicability of SE and PCE for treatment Cuddly Toy of oral HSV and possibly other viruses.